High-performance liquid chromatography (HPLC) is a useful analytical technique for separating, identifying, and quantifying the components of a mixture. It is essential in a wide range of scientific and industrial disciplines, from pharmaceutical to environmental and petrochemical analysis.
In this article, we’ll talk about what HPLC is, how it works, and its significance in the professional environment.
Understanding High-performance Liquid Chromatography
Put simply, HPLC is an approach used in the laboratory for separating and analysing complex chemical mixtures.
It uses a liquid mobile phase to transport a sample through a solid stationary phase-filled column and separates constituents depending on their interactions with the stationary phase. This technique is widely used in a variety of industries to quantify and identify substances in a mixture.
How Does It Work?
To conduct an HPLC test, the injector is loaded with the material to be tested, which is then forced through the column using a pump. Thereafter, the different parts of the sample elute at various rates as they move through the stationary phase.
The retention time is the amount of time it takes for each component to elute. This data, along with the detector response, is utilised to determine which substance is present and quantify the sample components.
Applications
HPLC is used in many industries, including pharmaceutics, where the active ingredients of drugs can be identified and quantified. It can also be used to determine which contaminants are present in environmental applications.
The food and beverage industry also makes use of this technique to determine whether pesticides are present.
Closing Remarks
The accuracy, sensitivity, and versatility of HPLC make it an essential instrument in modern analytical chemistry. It assists in quality control, research, and regulatory compliance across a wide range of industries.
If you would like to know more about our HPLC testing apparatus, please contact Trinity Biotech today!
